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twist sirna  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology twist sirna
    Twist Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/twist+sirna/twist+siRNA/pm41828479-211-43-48
    Average 94 stars, based on 33 article reviews
    twist sirna - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: DUSP4 promotes doxorubicin resistance in gastric cancer through epithelial-mesenchymal transition
    Article Snippet: Doxorubicin was obtained from Sigma (St. Louis, MO, USA) and dissolved in ddH2O. .. GC cells were transfected with DUSP4 siRNA (100 nmol/L; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or Twist siRNA (100 nmol/L; Santa Cruz) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. Non-specific negative control siRNA was purchased from GeneChem Co., Ltd. (Shanghai, China).

    Article Title: TBOPP, a DOCK1 Inhibitor, Potentiates Cisplatin Efficacy in Breast Cancer by Regulating Twist-mediated EMT
    Article Snippet: .. For the cell transfection assay, DOCK1 siRNA, Twist siRNA, or negative siRNA were synthesized by Santa Cruz Biotechnology (CA, USA). .. Twist plasmid was obtained from OriGene Company (MR227370).

    Article Title: Inhibition of AMPK-related kinase 5 (ARK5) enhances cisplatin cytotoxicity in non-small cell lung cancer cells through regulation of epithelial-mesenchymal transition
    Article Snippet: .. NSCLC cells were transfected with ARK5 siRNA (100 nM) or Twist siRNA (100 nM; Santa Cruz Biotechnology, Dallas, TX, USA) using Lipofectamine 2000 according to the manufacturer’s protocol. ..

    Article Title: Inhibition of AMPK-related kinase 5 (ARK5) enhances cisplatin cytotoxicity in non-small cell lung cancer cells through regulation of epithelial-mesenchymal transition
    Article Snippet: All cells were grown in RPMI 1640 supplemented with 10% fetal bovine serum (FBS; GIBCO, Grand Island, USA) and 1% penicillin/streptomycin (Sigma, St. Louis, MO, USA). .. Cells were maintained under 5% CO 2 at 37°C in a humidified incubator. siRNA transfections NSCLC cells were transfected with ARK5 siRNA (100 nM) or Twist siRNA (100 nM; Santa Cruz Biotechnology, Dallas, TX, USA) using Lipofectamine 2000 according to the manufacturer’s protocol. ..

    Article Title: Lactate Promotes Endothelial-Mesenchymal Transition via Mediating Twist1 Lactylation in Hypoxic Pulmonary Hypertension
    Article Snippet: .. To investigate the effects of lactate on HPAECs, the cells were exposed to lactate (concentration gradient 0–10 mM, 71718, Sigma-Aldrich, St. Louis, MO, USA) for 24 h. To examine the role of Twist in lactate-induced EndoMT, HPAECs at 70–80% confluency were transfected with Twist siRNA (100 nM, sc-38604, Santa Cruz, Dallas, TX, USA), with scrambled siRNAs serving as controls. ..

    Article Title: TBOPP, a DOCK1 Inhibitor, Potentiates Cisplatin Efficacy in Breast Cancer by Regulating Twist-mediated EMT.
    Article Snippet: Background: DOCK1 has been reported to be involved in tumor progression and resistance.1-(2-(30-(trifluoromethyl)-[1,10-biphenyl]-4-yl)-2-oxoethyl)-5-pyrrolidinylsulfonyl2(1H)pyridone (TBOPP) is a selective DOCK1 inhibitor; however, the role and molecular mechanisms of DOCK1 and its inhibition in breast cancer (BC) resistance remain poorly understood.. Objective: This study aims toinvestigate the underlying mechanisms of DOCK1 in BC resistance.. Methods: DOCK1 or Twist siRNA and Twist plasmid were used to explore the function of DOCK1 in vitro experiments.

    Synthesized:

    Article Title: TBOPP, a DOCK1 Inhibitor, Potentiates Cisplatin Efficacy in Breast Cancer by Regulating Twist-mediated EMT
    Article Snippet: .. For the cell transfection assay, DOCK1 siRNA, Twist siRNA, or negative siRNA were synthesized by Santa Cruz Biotechnology (CA, USA). .. Twist plasmid was obtained from OriGene Company (MR227370).

    Article Title: TBOPP, a DOCK1 Inhibitor, Potentiates Cisplatin Efficacy in Breast Cancer by Regulating Twist-mediated EMT.
    Article Snippet: Background: DOCK1 has been reported to be involved in tumor progression and resistance.1-(2-(30-(trifluoromethyl)-[1,10-biphenyl]-4-yl)-2-oxoethyl)-5-pyrrolidinylsulfonyl2(1H)pyridone (TBOPP) is a selective DOCK1 inhibitor; however, the role and molecular mechanisms of DOCK1 and its inhibition in breast cancer (BC) resistance remain poorly understood.. Objective: This study aims toinvestigate the underlying mechanisms of DOCK1 in BC resistance.. Methods: DOCK1 or Twist siRNA and Twist plasmid were used to explore the function of DOCK1 in vitro experiments.

    other:

    Article Title: Lactate Promotes Endothelial-Mesenchymal Transition via Mediating Twist1 Lactylation in Hypoxic Pulmonary Hypertension.
    Article Snippet: Lactate levels in plasma, or lung tissues were measured using a Lactate Assay Kit (ab65330, Abcam, Cambridge, UK).

    Small Interfering RNA:

    Article Title: Downregulation of DOCK1 sensitizes bladder cancer cells to cisplatin through preventing epithelial–mesenchymal transition
    Article Snippet: Cisplatin was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). .. The DOCK1 small interfering RNA (siRNA) and Twist siRNA were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). ccK-8 assay Cells at 3.0×103 cells/well were seeded into 96-well plates. .. Then, 10 μL/well Cell Counting Kit-8 (CCK-8) solution (Dojindo, Kumamoto, Japan) was added, the plates were incubated for 3 hours, and absorbance was measured at 450 nm using an MRX II microplate reader (Dynex, Chantilly, VA, USA). edU incorporation assay DNA incorporation synthesis was calculated using EdU (5-ethynyl-2-deoxyuridine) incorporation assay.

    CCK-8 Assay:

    Article Title: Downregulation of DOCK1 sensitizes bladder cancer cells to cisplatin through preventing epithelial–mesenchymal transition
    Article Snippet: Cisplatin was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). .. The DOCK1 small interfering RNA (siRNA) and Twist siRNA were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). ccK-8 assay Cells at 3.0×103 cells/well were seeded into 96-well plates. .. Then, 10 μL/well Cell Counting Kit-8 (CCK-8) solution (Dojindo, Kumamoto, Japan) was added, the plates were incubated for 3 hours, and absorbance was measured at 450 nm using an MRX II microplate reader (Dynex, Chantilly, VA, USA). edU incorporation assay DNA incorporation synthesis was calculated using EdU (5-ethynyl-2-deoxyuridine) incorporation assay.

    Concentration Assay:

    Article Title: Lactate Promotes Endothelial-Mesenchymal Transition via Mediating Twist1 Lactylation in Hypoxic Pulmonary Hypertension
    Article Snippet: .. To investigate the effects of lactate on HPAECs, the cells were exposed to lactate (concentration gradient 0–10 mM, 71718, Sigma-Aldrich, St. Louis, MO, USA) for 24 h. To examine the role of Twist in lactate-induced EndoMT, HPAECs at 70–80% confluency were transfected with Twist siRNA (100 nM, sc-38604, Santa Cruz, Dallas, TX, USA), with scrambled siRNAs serving as controls. ..



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    Image Search Results


    Sequences of siRNAs and PCR Primers

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Promotor Hypomethylation Mediated Upregulation of VCAN Targets Twist1 to Promote EndMT in Hypoxia‐Induced Pulmonary Hypertension

    doi: 10.1161/JAHA.124.036969

    Figure Lengend Snippet: Sequences of siRNAs and PCR Primers

    Article Snippet: Small interfering RNAs (siRNAs) targeting versican and Twist1 were purchased from RiboBio (Guangzhou, China).

    Techniques: Sequencing

    A and B , Representative images of immunohistochemical staining showed that Twist1 was upregulated in the pulmonary endothelium of patients with HPH compared with the normal group (n=3). Positive staining was indicated by a brown color and pointed out with black arrows. ×400, scale bar: 50 μm. * P <0.05. ( C and D ) The protein level and densitometric quantification of Twist1 in lung tissues of 2 groups were determined by Western blot analysis (n=3). * P <0.05. ( E and F ) Representative images of immunohistochemical staining showed that knocking down VCAN in vivo reduces upregulated Twist1 in the pulmonary vascular endothelium of HPH mice. Positive staining is indicated by a brown color and pointed out with black arrows (n=3). ×400, scale bar: 50 μm. * P <0.05, ** P <0.01. ( G–J ) The protein levels and densitometric quantification of Twsit1 in vivo and in vitro knockdown VCAN assays were determined by Western blot analysis (n=3). * P <0.05, ** P <0.01, *** P <0.001. K , The CO‐IP experiment showed that VCAN and twist1 can bind to each other in HPAECs. The target protein VCAN was immunoprecipitated with either anti‐Twist1 antibody or IgG. L , Enlarged images of the immunofluorescence staining of HPAECs nucleus. × 400, scale bar: 25 μm. An unpaired 2‐tailed Student t test was performed for comparisons between the normal group and patients with HPH, while ANOVA with the Tukey test was performed for comparison between 4 groups of in vivo and in vitro experiments. CO‐IP indicates Co‐Immunoprecipitation; EndMT, endothelial‐to‐mesenchymal transition; HPAECs, human pulmonary artery endothelial cells; HPH, hypoxia‐induced pulmonary hypertension; and VCAN, versican.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Promotor Hypomethylation Mediated Upregulation of VCAN Targets Twist1 to Promote EndMT in Hypoxia‐Induced Pulmonary Hypertension

    doi: 10.1161/JAHA.124.036969

    Figure Lengend Snippet: A and B , Representative images of immunohistochemical staining showed that Twist1 was upregulated in the pulmonary endothelium of patients with HPH compared with the normal group (n=3). Positive staining was indicated by a brown color and pointed out with black arrows. ×400, scale bar: 50 μm. * P <0.05. ( C and D ) The protein level and densitometric quantification of Twist1 in lung tissues of 2 groups were determined by Western blot analysis (n=3). * P <0.05. ( E and F ) Representative images of immunohistochemical staining showed that knocking down VCAN in vivo reduces upregulated Twist1 in the pulmonary vascular endothelium of HPH mice. Positive staining is indicated by a brown color and pointed out with black arrows (n=3). ×400, scale bar: 50 μm. * P <0.05, ** P <0.01. ( G–J ) The protein levels and densitometric quantification of Twsit1 in vivo and in vitro knockdown VCAN assays were determined by Western blot analysis (n=3). * P <0.05, ** P <0.01, *** P <0.001. K , The CO‐IP experiment showed that VCAN and twist1 can bind to each other in HPAECs. The target protein VCAN was immunoprecipitated with either anti‐Twist1 antibody or IgG. L , Enlarged images of the immunofluorescence staining of HPAECs nucleus. × 400, scale bar: 25 μm. An unpaired 2‐tailed Student t test was performed for comparisons between the normal group and patients with HPH, while ANOVA with the Tukey test was performed for comparison between 4 groups of in vivo and in vitro experiments. CO‐IP indicates Co‐Immunoprecipitation; EndMT, endothelial‐to‐mesenchymal transition; HPAECs, human pulmonary artery endothelial cells; HPH, hypoxia‐induced pulmonary hypertension; and VCAN, versican.

    Article Snippet: Small interfering RNAs (siRNAs) targeting versican and Twist1 were purchased from RiboBio (Guangzhou, China).

    Techniques: Immunohistochemical staining, Staining, Western Blot, In Vivo, In Vitro, Knockdown, Co-Immunoprecipitation Assay, Immunoprecipitation, Immunofluorescence, Comparison

    The primer sequences used for the qRT-PCR analysis

    Journal: Human Cell

    Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

    doi: 10.1007/s13577-024-01087-6

    Figure Lengend Snippet: The primer sequences used for the qRT-PCR analysis

    Article Snippet: The ADSCs were plated onto 12-well plates and transduced with either 12 ml of LV particles containing shRNA targeting TWIST1 (sc-38604-V; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or LV particles containing scramble shRNA (sc-108080; Santa Cruz Biotechnology) each well in DMEM added with GlutaMAX plus 5 mg/ml polybrene for 6 h to achieve TWIST1 knockdown.

    Techniques: Sequencing

    Characterization of ADSCs and their differentiation into Schwann cells. A, Phenotypic characterization of ADSCs by flow cytometric analysis. B, The qRT-PCR analysis of TWIST1 mRNA expression levels in ADSCs 2 weeks after LV transduction. C, Immunoblotting analysis of TWIST1 protein expression levels in ADSCs 2 weeks after LV transduction. * P < 0.05 compared to the scramble siRNA by unpaired t tests. D, Representative inverted microscope images of ADSCs under Schwann cell induction for 14 days. Without induction, ADSCs showed a mesh-like structure. After 14 days, cells adopted a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern, resembling Schwann cells. Notably, the sh-TWIST1 group exhibited more pronounced Schwann cell-like features than the scramble group

    Journal: Human Cell

    Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

    doi: 10.1007/s13577-024-01087-6

    Figure Lengend Snippet: Characterization of ADSCs and their differentiation into Schwann cells. A, Phenotypic characterization of ADSCs by flow cytometric analysis. B, The qRT-PCR analysis of TWIST1 mRNA expression levels in ADSCs 2 weeks after LV transduction. C, Immunoblotting analysis of TWIST1 protein expression levels in ADSCs 2 weeks after LV transduction. * P < 0.05 compared to the scramble siRNA by unpaired t tests. D, Representative inverted microscope images of ADSCs under Schwann cell induction for 14 days. Without induction, ADSCs showed a mesh-like structure. After 14 days, cells adopted a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern, resembling Schwann cells. Notably, the sh-TWIST1 group exhibited more pronounced Schwann cell-like features than the scramble group

    Article Snippet: The ADSCs were plated onto 12-well plates and transduced with either 12 ml of LV particles containing shRNA targeting TWIST1 (sc-38604-V; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or LV particles containing scramble shRNA (sc-108080; Santa Cruz Biotechnology) each well in DMEM added with GlutaMAX plus 5 mg/ml polybrene for 6 h to achieve TWIST1 knockdown.

    Techniques: Quantitative RT-PCR, Expressing, Transduction, Western Blot, Inverted Microscopy

    The sciatic nerves of rats in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were collected and subjected to histomorphological analysis. A, The sections of rat sciatic nerve stained with toluidine blue; the non-lesioned rats showed homogeneously distributed fibers, uniform myelin sheath, and normal axon diameter; the non-transplanted rats showed clear signs of nerve fiber degeneration with presence of myelin degradation, indicated by white arrows; the LV-scramble-ADSC rats showed some signs of regenerated nerve fibers but presence of degenerated axons and decreased myelin sheath thickness; the LV-sh-TWIST1-ADSC rats showed clear signs of regenerated nerve fibers surrounded by newly formed myelin sheaths. B, Morphometric analysis of axion diameter and myelin sheath thickness. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

    Journal: Human Cell

    Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

    doi: 10.1007/s13577-024-01087-6

    Figure Lengend Snippet: The sciatic nerves of rats in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were collected and subjected to histomorphological analysis. A, The sections of rat sciatic nerve stained with toluidine blue; the non-lesioned rats showed homogeneously distributed fibers, uniform myelin sheath, and normal axon diameter; the non-transplanted rats showed clear signs of nerve fiber degeneration with presence of myelin degradation, indicated by white arrows; the LV-scramble-ADSC rats showed some signs of regenerated nerve fibers but presence of degenerated axons and decreased myelin sheath thickness; the LV-sh-TWIST1-ADSC rats showed clear signs of regenerated nerve fibers surrounded by newly formed myelin sheaths. B, Morphometric analysis of axion diameter and myelin sheath thickness. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

    Article Snippet: The ADSCs were plated onto 12-well plates and transduced with either 12 ml of LV particles containing shRNA targeting TWIST1 (sc-38604-V; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or LV particles containing scramble shRNA (sc-108080; Santa Cruz Biotechnology) each well in DMEM added with GlutaMAX plus 5 mg/ml polybrene for 6 h to achieve TWIST1 knockdown.

    Techniques: Transplantation Assay, Staining

    The mRNA expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the qRT-PCR analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

    Journal: Human Cell

    Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

    doi: 10.1007/s13577-024-01087-6

    Figure Lengend Snippet: The mRNA expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the qRT-PCR analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

    Article Snippet: The ADSCs were plated onto 12-well plates and transduced with either 12 ml of LV particles containing shRNA targeting TWIST1 (sc-38604-V; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or LV particles containing scramble shRNA (sc-108080; Santa Cruz Biotechnology) each well in DMEM added with GlutaMAX plus 5 mg/ml polybrene for 6 h to achieve TWIST1 knockdown.

    Techniques: Transplantation Assay, Quantitative RT-PCR

    The protein expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the immunoblotting analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

    Journal: Human Cell

    Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

    doi: 10.1007/s13577-024-01087-6

    Figure Lengend Snippet: The protein expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the immunoblotting analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

    Article Snippet: The ADSCs were plated onto 12-well plates and transduced with either 12 ml of LV particles containing shRNA targeting TWIST1 (sc-38604-V; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or LV particles containing scramble shRNA (sc-108080; Santa Cruz Biotechnology) each well in DMEM added with GlutaMAX plus 5 mg/ml polybrene for 6 h to achieve TWIST1 knockdown.

    Techniques: Transplantation Assay, Western Blot